Dual-Action Compounds Targeting Adenosine A2A Receptor and Adenosine Transporter for Prevention and Treatment of Neurodegenerative Diseases

ABSTRACT

The present invention provides therapeutic agents for preventing and treating neurodegenerative diseases. These agents synergistically target both the adenosine A 2A  receptor (A 2A R) and the equilibrative nucleoside transporter 1 (ENT1).

The present application claims priority to U.S. Provisional Patent Application 61/260, 932, filed Nov. 13, 2010, the contents of which is hereby incorporated in its entirety by reference.

TECHNICAL FIELD

The present invention provides the identification, synthesis and use of compound active for treating neurodegenerative diseases, such as Huntington's disease.

Abbreviations: A_(2A)R, A_(2A) adenosine receptor; Ac₂O, acetic anhydride; CGS, 6-amino-9-(5-ethylcarbamoyl-3,4-dihydroxy-oxolan-2-yl)-2-{2-[4-(2-carboxyethyl)phenyl]ethylamino}purine; DIEA, diisopropylethylamine; DMF, N,N-dimethylformamide; DML, designed multiple ligands; ENT, equilibrative nucleotide transporter, ESI, electrospray ionization; EtOAc, ethyl acetate; HD, Huntington's disease; hENT1, human equilibrative nucleoside transporter 1; HBA, hydrogen bond acceptor; HBD, hydrogen bond donor; HBTU. 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium; HOBt, 1-hydroxybenzotriazole; HP, hydrophobic; HPLC, high-performance liquid chromatography; IR, infrared; JNK, c-Jun N-terminal kinase; MS, mass spectrometry; MsCl, methanesulfonyl chloride; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NBTI, S-(4-nitrobenzyl)-6-thioinosine; NMR, nuclear magnetic resonance; py, pyridine; PyBOP, benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate; RA, ring aromatic; t-BuOH, tertiary butanol; TEMPO, 2,2,6,6-tetramethylpiperidinyl-1-oxy; THF, tetrahydrofuran; TLC, thin-layer chromatography; TsCl, p-toluenesulfonyl chloride; TsOH, p-toluenesulfonic acid; ZM, 4-(2-[7-Amino-2-{2-furyl}{1,2,4}triazolo{2,3-a}{1,3,5}triazin-5-yl-amino]ethyl)phenol.

BACKGROUND ART Introduction

Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by a CAG trinucleotide expansion in the Huntingtin (Htt) gene, which shows chorea, dementia, and psychiatric symptoms.¹⁻³ Effective treatment for HD has not yet been developed, though a few therapeutic agents with moderate effects have been reported.^(4,5) It has been demonstrated that the selective A_(2A) adenosine receptor (A_(2A)R) agonist, CGS21680 (in short, CGS), can attenuate the HD symptoms in a transgenic mouse model,⁶ and this compound has been shown to be able to rescue the urea cycle deficiency of HD disease by enhancing the activity of the ubiquitin-proteasome system.⁷ However, CGS is known to exert strong immunosuppressive effect,⁸ among other side effects, and therefore is not suitable for clinical use. On the other hand, an adenosine analogue, designated as T1-11 (compound 1), also an A_(2A)R agonist, is recently isolated from Gastordia elata and shown to prevent serum-derived PC12 cell apoptosis, suggesting its therapeutic potential in treating neural degenerative diseases.⁹

SUMMARY OF THE INVENTION

It has been recognized that the A_(2A)R and the adenosine transporter (such as the equilibrative nucleoside transporter ENT1) are both localized in the striatum,¹⁰⁻¹³ where the mutant Htt aggregate.¹⁴ Inhibition of the adenosine transporter would elevate the local concentrations of adenosine, and thereby increases the efficacy in agonizing the A_(2A)R. Interestingly, compound 1 is also found to be an ENT1 inhibitor. On the other hand, the potent immunosuppressant effect of CGS was a consequence of A_(2A)R signaling,⁸ which indicates that extremely strong binding affinity to A_(2A)R may not be a desirable property for clinically useful therapeutic agents.

It is therefore of considerable interest to modify compound 1 yet to retain its multiple-action property. Besides, transient or weakly binding compounds may be preferable than stably binding compounds,¹⁵ especially when targeting the A_(2A)R signaling system due to the aforementioned reasons. Designed multiple ligands (DML)^(16,17) are conceptually distinct from promiscuous compounds discovered by random screening, because they are rationally designed to optimize the desired properties. It has been proposed that DMLs may be advantageous for treatment of diseases with complex etiologies, e.g., asthma, obesity, cancer, and psychiatric diseases, compared to the individually-targeting compounds.¹⁶⁻¹⁸ It has been conceived that the intrinsic redundancy and robustness of complex biological networks may be responsible to the failure of highly selective drugs to deliver the intended therapeutic effects.¹⁹ A recent assessment indicates that A_(2A)R pharmacology is indeed rather complex,²⁰ and therefore DMLs may be especially suitable for targeting the A_(2A)R signaling system. However, to design multiple-targeting ligands is often a challenging task,^(21,22) partly due to the difficulty in appropriate construction of the computational models for describing the interactions of the ligands with several targets, and partly due to the increased restraints of chemical synthesis, and satisfaction of physical chemical properties of compounds. Perhaps associated with the increasing complexity as the number of targets increases, currently most of reported DMLs are dual-function ligands.²²

We have described in our previously filed provisional application, U.S. Provisional Patent Application 61/260,932, a number of compounds that have been synthesized and that exhibit dual functions on both adenosine receptors and transporters. These are generally described as compounds having an adenosine structural scaffold with variations at C-6 and C-5′ with the following structure

wherein n is 1 to 3, R¹ selected from the group consisting of (substituted)-benzene, polyarene and heterocycle, R² is selected from the group consisting of halogen, hydroxyl, alkoxy, azido, amino, (substituted)amino, amido, sulfanyl, sulfonyl, triazolyl, and cyano groups, and R³ is selected from the group consisting of (substituted) carbonyl, carboxylate, (substituted) carbamide, cyano, (substituted)alkynyl, and (substituted) tetrazole groups.

Preferably, the compounds of one embodiment of the present invention have the structure of

wherein the (substituted) heterocycle contains 5- or 6-membered rings and the fused heterocycle contain nitrogen, oxygen or sulfur heteroatoms and the substituents is selected from the group consisting of hydrogen, halogen (fluorine, chlorine, bromine and iodine), hydroxy, alkyl (1 to 6 carbons), trifluoromethyl, and (substituted)phenyl group.

The heterocycle may be pyrrole, furan, thiophene, pyridine, piperidine, piperazine, indole, benzofuran, benzothiophene, or quinoline.

Another preferable embodiment are compounds having the structure of

Wherein the aromatic ring is selected from the group consisting of benzene, naphthalene, anthracene, phenanthrene, and pyrene; when n=1, the benzyl group may optionally have substituents selected from the group consisting of halogen (fluorine, chlorine, bromine and iodine), alkyl (methyl, ethyl, propyl, butyl and trifluoromethyl), phenyl, hydroxy, alkoxyl (OR where R═CH₃, C₂H₅, C₃H₇ and C₄H₉), amino (NRR′ where R, R′ represent H, CH₃, C₂H₅, C₃H₇, C₄H₉ and phenyl), amido (NHCOR where R═CH₃, C₂H₅, C₃H₇ and C₄H₉), nitro, sulfonate, alkanoyl (COR where R═H, CH₃, C₂H₅, C₃H₇, C₄H₉ and phenyl), and carboxylate (CO₂R where R═H, CH₃, C₂H₅, C₃H₇, C₄H₉ and phenyl).

Preferably, the compound has the structure of

wherein R³ is selected from the group consisting of hydrogen, alkyl (1 to 4 carbons), and (substituted)phenyl groups, and R¹ is as described above.

Another preferable compound has the structure of

wherein R⁴ is selected from the group consisting of hydrogen, halogen (fluorine, chlorine, bromine and iodine), hydroxy, alkyl (1 to 6 carbons), trifluoromethyl, and (substituted)phenyl group, and R¹ is as described above.

Still another preferable compound has the structure of

wherein R⁵ is selected from the group consisting of hydrogen and alkyl (1 to 4 carbons) groups, and R¹ is as described above.

Another preferable compound has the structure of

wherein R is selected from the group consisting of H, halogen (F, Cl, Br, and I), alkyl (1 to 4 carbons, trifluoromethyl, phenyl, hydroxyl, alkoxy (1 to 4 carbons), (substituted)amino, (substituted)amido, nitro, sulfonate, carbonyl, and carboxylate groups located at ortho-, meta- or para-positions, and wherein x is 1 to 5.

The present invention, in another embodiment, provides a method for treating neurodegenerative disease, comprising administering to a subject in need thereof an effective amount of at least one of the compounds discussed above. The neurodegenerative disease may be a protein-misfolding disease which is defined as a disease caused by protein-misfolding. These protein-misfolding diseases include Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Prion disease, Huntington's disease, and spinal Cerebellar ataxias. The present method is especially useful in treating Huntington's disease.

As another embodiment, the present invention provides for a composition comprising an effective amount of at least one of the above described compounds and a pharmaceutically acceptable carrier.

To rationally design the proper dual-action ligands as therapeutics for Huntington's disease, we first constructed two pharmacophore models, one for the A_(2A)R agonists, and the other for the ENT1 inhibitors. Three-dimensional pharmacophores are specific spatial distributions of chemical functional features of a series of compounds that target the same active site of a biomolecule and exert the same function,²³ which are particular useful if the high resolution X-ray or NMR structures of the target biomolecules are not yet available. Pharmacophore analysis has been successfully applied to numerous drug discovery tasks.²⁴⁻²⁹ Although the high resolution crystal structure of human adenosine A_(2A) receptor has been released recently,³⁰ it represents the conformation bound with the antagonist ZM241385 (in short, ZM), not with an agonist. On the other hand, the crystal structure of human ENT1 (hENT1) is not yet available, and there is also no suitable structural template for homology modeling.

Based on the structural scaffold of 1, we set out to design a series of adenosine derivatives (FIG. 1). Chemical modifications of adenosine were carried out if the pharmacophore fitting of the modified compound predicts acceptable activity. The competitive ligand binding assays were performed to verify if the designed compounds indeed bind to the A_(2A)R and ENT1 with good affinity. Finally, these compounds were assayed whether they could prevent apoptosis of the serum-deprived PC12 cells, which is a crucial indication for their potential for treating neurodegenerative diseases.^(31,32)

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. Structures of CGS, NBTI and some designed adenosine derivatives with modification at the N⁶- and C⁶′-positions.

FIG. 2. The 3D pharmacophore model of the adenosine A_(2A) receptor agonists. (A) The geometric features of the pharmacophore model. Cyan: hydrophobic (HP), gold: ring aromatic (RA), magenta: hydrogen bond donor (HBD), green: hydrogen bond acceptor (HBA) (B) Fitting of CGS into the pharmacophore model. (C) Fitting of compound 1. (D) Fitting of NBTI. (E) Fitting of compound 6. (F) Fitting of compound 11.

FIG. 3. The 3D pharmacophore model of the human equilibrative nucleoside transporter (hENT1) inhibitors. (A) The geometric features of the pharmacophore model. Gold: ring aromatic (RA), green: hydrogen bond acceptor (HBA1: 3.431 Å distance from RA; HBA2: 10.388 Å distance from RA). (B) Fitting of NBTI into the pharmacophore model. (C) Fitting of compound 1. (D) Fitting of CGS. (E) Fitting of compound 6. (F) Fitting of compound 11.

FIG. 4. The scatter plot of the predicted pK_(i) values of A_(2A)-R agonists versus the measured pK_(i) values. The filled circles represent the training compounds, and the open circles the synthesized compounds.

FIG. 5. The scatter plot of the predicted pK_(i) values of ENT1 inhibitors versus the measured pK_(i) values. The filled circles represent the training compounds, and the open circles the synthesized compounds.

DESCRIPTION OF THE EMBODIMENTS Pharmacophore Model of the Human Adenosine A_(2A) Receptor Agonists

As part of the dual-pharmacophore drug design approach, a 3D-pharmacophore model of the human A_(2A)R (hA_(2A)R) agonists was first constructed to design the compounds that could function as hA_(2A)R agonists. The training set includes 25 compounds having large range of structural diversity and hA_(2A)R activity (K_(i) from 1.2 nM to 187 μM) selected from the literature. A potent hA_(2A)R agonist CGS,³³ is also included in this training set. The HypoGen® module of Catalyst® of Accelrys® ³⁴ was used to construct the pharmacophore model of these ligands. The constructed pharmacophore is illustrated in FIG. 2A, which shows four geometric features including hydrophobic (HP, in cyan), ring aromatic (RA, in gold), hydrogen bond donor (HBD, in magenta) and hydrogen bond acceptor (HBA, in green). For CGS all the four features of the constructed pharmacophore can be fitted nicely (FIG. 2B). In contrast, S-(4-nitrobenzyl)-6-thioinosine (NBTI)³⁵ lacks a ring-aromatic fitting (FIG. 2D), in agreement with its weak affinity as a hA_(2A)R ligand, though it exhibits a strong binding with adenosine transporter. The designed dual-action ligands 1, 6 and 11 fit at least three features in this pharmacophore model for A_(2A)R agonists (FIGS. 2C, 2E and 2F).

Pharmacophore Model of the Equilibrative Nucleoside Transporter Inhibitors.

To design dual-function compounds that act cooperatively as the hA_(2A)R agonists and the hENT1 inhibitors, the pharmacophore of hENT1 inhibitors was also constructed (FIG. 3A). The training set includes 25 compounds possessing hENT1 inhibitory activity ranging from IC₅₀ of 0.29 nM to 32 μM, which were selected from the literature (see Supporting Information). The constructed pharmacophore model of the hENT1 inhibitors consists of only three features, including two hydrogen bond acceptors and one ring aromatic. All the five compounds (NBTI, 1, CGS, 6, and 11) can fit into all these three features (FIGS. 3B-F). The different number of features between the pharmacophores of hA_(2A)R agonists and hENT1 inhibitors could be attributed to the nature of the training set compounds.

By carefully scrutinizing the structures in the hA_(2A)R agonists training set (see Supporting Information), we found that many of them possess a hydrophobic group in the 5′ end of nucleoside, especially those compounds with higher potency, including CGS. Therefore, the constructed pharmacophore must include this important feature. On the contrary, in the hENT1 inhibitors training set, almost all the 5′ end of the nucleoside possesses a polar hydroxyl group.

The pharmacophore differences among the two investigating targets also shed some light on the design of dual-function compounds. For example, compound 6 does not fit well to the hydrophobic feature in A_(2A) receptor pharmacophore model, and it is indeed less potent than CGS, which fits well to all features. However, compound 6 could fit well in all three features in hENT1 model.

As for compound 11, it fits well to all features including hydrophobic of A_(2A)R pharmacophore mode. Nevertheless, it still fits well to hENT1 pharmacophore model, indicating the higher tolerance of ENT1 pharmacophore model. In summary, by comparing the features and compound-fitting qualities of these two pharmacophore models, we may hypothesize that the hA_(2A)R binding pocket has an important hydrophobic site, and the hENT1 binding pocket may be more flexible to accommodate the nucleosides with hydrophobic moieties at the 5′ end in this series of compounds. Pharmacophore analyses provide us with an insight into the design and understanding of dual-function compound in the absence of the structural information of hENT1.

Synthesis of Adenosine Derivatives.

A representative library of adenosine analogues (FIG. 1) was developed based on the pharmacophore models. Compound 1, originally isolated from Gastrodia elata, ⁹ was synthesized in a high yield by the substitution reaction of 6-chloropurine ribofuranoside (17) with 4-hydroxybenzylamine (as the hydrochloric salt) in the presence of a base of diisopropylethylamine. As 4-hydroxybenzylamine was not commercially available, it was prepared in two steps from 4-hydroxybenzaldehyde via the condensation reaction with hydroxylamine to give an intermediate oxime, which was subsequently hydrogenated by the catalysis of Pd/C and HCl. By the procedures similar to that for compound 1, a series of N⁶-substituted adenosine derivatives 2-6 were prepared by the substitution reactions of 6-chloropurine ribofuranoside with appropriate substituted benzylamines. In lieu of the conventional heating method, microwave irradiation was also applied to shorten the reaction time in the preparation of N⁶-(3-indolylethyl)adenosine (6).

Treatment of N⁶-(4-methoxybenzyl)adenosine 3 with 2,2-dimethoxypropane in anhydrous acetone afforded the corresponding 3-acetonide, which reacted with p-toluenesulfonyl chloride in the presence of pyridine to give a mixture of tosylate and chloride derivatives (Scheme 1). The tosylate derivative was unstable, whereas the chloride compound (7-acetonide) could be isolated by chromatography and subsequently hydrolyzed to give 7. Without separation, this mixture was treated with sodium azide, followed by acid-catalyzed hydrolysis, to give an azido compound 8. Alternatively, Staudinger reduction of 8-acetonide gave an intermediate amine, which was converted to the corresponding acetamide 9 and sulfonamide 10 after removal of the 2′,3′-isopropylidene group in acid.

In another approach (Scheme 2), the Cu⁺-catalyzed 1,3-dipolar cycloaddition (click reaction)³⁸ of azido compound 8 with 1-hexyne, 1-octyne, and 3-phenyl-1-propyne afforded the triazole derivatives 12, 13 and 14, respectively. Likewise, the click reaction of 8-acetonide with propargyl alcohol gave a triazole compound, of which hydroxyl group was activated as a mesylate and then reduced by NaBH₄ to give a methyl group. Compound 11 was obtained after removal of the 2′,3′-isopropylidene group.

The acetonide of 6-chloropurine ribofuranoside was oxidized by (diacetoxyiodo)benzene with catalysis of 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO)³⁹ to give a carboxylic acid 18. The coupling reactions of acid 18 with ethylamine and ammonia gave amides 19 and 20, respectively. The chlorine atom in 19 was replaced by 4-hydroxybenzylamine, and the subsequent hydrolysis of the acetonide furnished amide 15. On the other hand, amide 20 was converted to nitrile 21 on treatment with Me₂SO, oxalyl chloride and i-Pr₂NEt.⁴⁰ After the chlorine atom was substituted by 4-methoxybenzylamine, a 1,3-dipolar cycloaddition of the cyano group with NaN₃ introduced the desired tetrazole moiety at the C-5′ position,⁴¹ giving 16 after removal of the 2′,3′-isopropylidene group under acidic conditions.

Biological Evaluation of N⁶- and C⁵-Modified Adenosine Derivatives.

The pharmacological properties of the prepared adenosine analogues were characterized by MDS Pharma Services using radioligand binding assays. The binding constants (K_(i)) of some representative compounds are shown in Table 1. The potent A_(2A)R agonist CGS appears to lack the activity against ENT1, whereas the ENT1 inhibitor NBTI shows no binding ability with A_(2A)R. Neither CGS nor NBTI is dual-functional drug. Some prepared adenosine analogues exhibit the dual actions on A_(2A)R and ENT1, in particular, compounds 1, 4 and 6 showing the K_(i) values in low micromolar and sub-micromolar range with A_(2A)R and ENT1, respectively. Except for 11 and 15, the adenosine derivatives having modification at the C-5′ position appeared to deteriorate their binding with A_(2A)R, though they still maintained high affinity with ENT1.

TABLE 1 Binding activity of the N⁶- and C⁵′-modified adenosine derivatives with adenosine receptor and transporter.^(a) K_(i) (μM) Compound A_(2A)-R^(b) ENT1^(c) CGS 7.77 × 10⁻² — NBTI >10  2.9 × 10⁻⁴ 1 2.62 5.38 × 10⁻¹ 1-acetonide >100 >100 2 14.4 1.44 × 10⁻² 3 30.1 3.18 × 10⁻¹ 4 3.21 3.72 5 27.7 6.51 × 10⁻³ 6 4.39 3.47 7 >100 2.98 8 — 5.81 × 10⁻¹ 9 >100 1.43 10  >100   2 × 10⁻¹ 11  41.8 9.60 × 10⁻¹ 12  >100 5.11 × 10⁻¹ 13  >100  5.2 × 10⁻² 14  >100 1.16 × 10⁻¹ 15  20.3 >10 16  >100 1.17 ^(a)The radioligand binding assays were performed by MDS Pharma Services Taiwan (Taipei, Taiwan) using standard binding protocols. ^(b)Human adenosine A_(2A) receptor. ^(c)Guinea pig equilibrium transporter 1.

We have previously reported that compound 1 isolated from an aqueous methanolic extract of Gastrodia elata prevented apoptosis of serum-deprived PC12 cells by suppressing LINK activity.¹⁵ In this study, serum-deprived PC12 cells were treated with the compound at the indicated dose for 24 h. Cell viability was monitored by the MTT assay, and is expressed as a percentage of the MTT activity measured in the serum-containing group. At a concentration of 0.01 μM, compounds 4 and 6 also rescued PC12 cells from the apoptosis evoked by serum withdrawal equally as well as 1 according to the cell viability of MTT assays. Collectively, the dual function of these compounds in activation of adenosine receptor and in inhibition of adenosine transporter might synergistically increase the effective concentration of adenosine, especially when these two proteins are in proximity.

Statistical Assessment of Pharmacophore Models.

FIG. 4 shows the scatter plot of the experimental pK_(i) versus predicted pK_(i) values from the pharmacophore model of A_(2A)R agonists. The r² value of the predicted K_(i) values versus the experimental K_(i) values is 0.962, and the root-mean-square of error (rmse) is 0.658 kcal/mol. This pharmacophore model was further evaluated using the Fisher's randomization test for statistical significance, as implemented in the CatScramble module. The CatScrambler module scrambled the pK_(i) values randomly for 19 times to generate new hypotheses (i.e., pharmacophore models). None of the 19 hypotheses from the scrambled data had a cost lower than the reported hypothesis. Table 2 summarizes the fitted features of the compounds in FIG. 4, along with the distance deviation of the fitted location of the feature on the compound from the center of the feature in the pharmacophore model. To reiterate, Table 2 is a quantitative representation of FIG. 4. When a ligand is fitted into a pharmacophore, the quality of fitting (or mapping) is indicated by the “fit value.” A higher fit value represents a better fit, and the computer fit values depends on two factors: the weights assigned to the pharmacophore features and how close the features in the molecules are to the exact locations of the features in the pharmacophore model.

TABLE 2 Comparison of activities of compounds with the fitted number of features of the A_(2A)-R agonist pharmacophore model. The numbers are in the unit of Å. Fit Compound ID HBD HBA RA HP Value CGS21680 √/0.166 √/0.125 √/0.201 √/0.436 10.6482 1 (T1-11) √/0.121 √/0.135 √/0.248 x 8.68049 NBTI √/0.422 √/0.304 x √/0.901 8.62848  6 √/0.377 √/0.359 √/0.403 x 8.52907 11 √/1.181 √/0.445 √/0.434 √/0.544 9.59155

The potent A_(2A)R agonist CGS fits all the four features of the constructed pharmacophore, and the deviations of the fitted feature locations from the exact locations of the features of the pharmacophore model are also very small. Compared to CGS, compound 1 lacks a hydrophobic moiety to fit into the feature of the pharmacophore model, and therefore exhibited a reduced affinity. In contrast, NBTI lacks a ring-aromatic moiety, which completely abolishes the activity to agonize the adenosine A_(2A) receptor. This indicates that the ring-aromatic feature may be more important than the hydrophobic moiety in this pharmacophore model. Compound 6 does not fit the hydrophobic feature of the pharmacophore model, while compound 11 does show higher fit value. In contrast, the binding assays indicate that compound 6 (K_(i)=4.39 μM) exhibits ˜10-fold stronger binding affinity than compound 11 (K_(i)=41.8 μM). This can be rationalized by the fact that all the fitted locations of the features of the compound 6 have less deviations from the exact locations of the features.

The constructed pharmacophore model of the hENT1 inhibitors consists of only three features, namely, a ring aromatic feature and two hydrogen bond acceptors. The r² value of the predicted K_(i) values versus the experimental K_(i) values is 0.927, and the rmse is 0.85 kcal/mol (FIG. 5). This pharmacophore model was further evaluated by the CatScrambler module. All the five compounds (NBTI, 1, CGS, 6, and 11) can fit into all these three features (FIGS. 3B-F), and therefore the deviations from the exact locations of the features need to be compared (Table 3). Apparently, the most potent inhibitor, NBTI, has the highest fit value and smallest deviation of all three features. The fit values of compounds 1, 11 and 6 are 6.61, 6.4 and 5.86, respectively, which are consistent with the ranking of their measured activity. CGS (with a high fit value 7.1) is obviously an outlier of this model, since this compound has no inhibitory activity toward hENT1. However, CGS is the only compound with a ring aromatic feature fitted on to the nucleoside moiety (FIG. 3). It is thus important to carefully examine whether the fitted functional group is indeed the same as the functional groups of the training set compounds that define the consensus feature in the pharmacophore analysis. The “fit value” alone can not be considered the measure of fitness.

TABLE 3 Comparison of activities of compounds with the fitted number of features of the ENT1 inhibitor pharmacophore model. The numbers are in the unit of Å. Compound ID HBD HBA RA Fit Value NBTI √/0.421 √/0.647 √/0.793 5.92748  1 √/0.544 √/0.619 √/0.586 4.94133 CGS21680 √/0.567 √/1.276 √/0.358 5.37611  6 √/0.421 √/0.647 √/0.493 5.40138 11 √/0.74  √/0.588 √/0.942 4.35311

CONCLUSION

We have adopted a dual-pharmacophore modeling approach to design dual-action compounds targeting the A_(2A)R signaling system. Based on the structural scaffold of 1, we designed and synthesized a series of adenosine derivatives and carried out chemical modifications of adenosine if the pharmacophore fitting of the modified compound predicts acceptable activity. The competitive ligand binding assays verified that the designed compounds indeed bind to both A_(2A)R and ENT1 with moderate affinity. The effective amount of the designed compounds for therapeutic treatment of neurodegenerative diseases, including Huntington's disease, is 1.5-2.5 mg/kg, based on oral dosage of the representative T1-11 in mice. The preferred route of administration of the designed compounds is oral administration, either in immediate release or slow release forms. Finally, these compounds were shown to prevent apoptosis of the serum-deprived PC12 cells, which is a crucial indication for their potential for treating neurodegenerative diseases.

Experimental Section

Materials and Methods

All reagents and solvents were of reagents grade and were used without further purification unless otherwise specified. Tetrahydrofuran and diethyl ether were distilled from Na/benzophenone and CH₂Cl₂ was distilled from CaH₂. All air or moisture sensitive experiments were performed under argon. All glasses were dried in oven for more than 2 hours and used after cooling to room temperature in desiccators. Microwave reactions were conducted using a focused single mode microwave unit (CEM Discover). The machine consists of a continuous focused microwave power delivery system with operator selectable power output.

Melting points were recorded on a Yanaco micro apparatus. Optical rotations were measured on digital polarimeter of Japan JASCO Co. DIP-1000. [α]_(D) values are given in units of 10⁻¹ deg cm² g⁻¹. Infrared (IR) spectra were recorded on Nicolet Magna 550-II. NMR spectra were obtained on Varian Unity Plus-400 (400 MHz) and chemical shifts (δ) were recorded in parts per million (ppm) relative to δ_(H) 7.24/δ_(C) 77.0 (central line of t) for CHCl₃/CDCl₃, δ_(H) 2.05/δ_(C) 29.92 for (CH₃)₂CO/(CD₃)₂CO, δ_(H) 3.31/δ_(C) 49.0 for CH₃OH/CD₃OD, and δ_(H) 2.49 (m)/δ_(C) 39.5 (m) for (CH₃)₂SO/(CD₃)₂SO. The splitting patterns are reported as s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet) and br (broad). Coupling constants (J) are given in Hz. The ESI-MS experiments were conducted on a Bruker Daltonics BioTOF III high-resolution mass spectrometer. Analytical thin-layer chromatography (TLC) was performed on E. Merck silica gel 60 F₂₅₄ plates (0.25 mm). Compounds were visualized by UV, anisaldehyde or ninhydrin spray. Column chromatography was carried out on columns packed with 70-230 mesh silica gel. Purity of compounds tested on A_(2A)-R and ENT1 was assessed to be ≧95% by HPLC (Agilent HP-1100) with detection at 280 nm wavelength.

Construction of Pharmacophore Models.

The HypoGen module of Catalyst® of Accelrys was used to construct the pharmacophore model of human A_(2A)R agonists and human ENT 1 inhibitors. The chemical structures of the training set compounds and their binding affinities to the human A_(2A)R (or human ENT 1) were collected from the literature (see Supporting Information).⁴¹⁻⁴⁴ It is important to ensure that the activity of the training compounds cover at least four orders of magnitude, with at least three compounds in each log scale.^(23,45) It is also recommended to select compounds with larger chemical diversity as the training set.⁴⁵ Each compound was sketched with ChemDraw, and then imported into Catalyst 4.11. The “Best” option was used for conformation generation. In Catalyst®, the Poling algorithm was used to generate 250 conformations, whose energies are less than 20.0 kcal/mol from the lowest energy of all the conformations. Five molecular features were selected, namely, hydrophobic (HPh), hydrogen bond acceptor (HBA), hydrogen bond donor (HBD), positively ionizable atom (PI), and negatively ionizable atom (NI). All these compounds were loaded into the Catalyst's spreadsheet and the default uncertainty of 3 were assigned. All other parameters are as default.

N⁶-(4-Hydroxybenzyl)adenosine (1)⁹

Hydroxylamine hydrochloride (1.29 g, 18.6 mmol) and sodium acetate (1.67 g, 20.4 mmol) were added to a solution of 4-hydroxybenzaldehyde (1.25 g, 10.2 mmol) in ethanol (20 mL). The reaction mixture was stirred at room temperature for 6 h. Ethanol was removed under reduced pressure. The residue was added water, and then extracted with Et₂O (3×). The combined organic layer was dried over MgSO₄. After the volatiles were removed by rotary evaporation under reduced pressure, the residue was recrystallized from CH₂Cl₂ to give the oxime of 4-hydroxybenzaldehyde (1.3 g, 93%). C₇H₇NO₂; light yellow solid, mp 92.0-93.6° C.

A solution of the above-prepared oxime (342 mg, 2.5 mmol) and concentrated hydrochloric acid (1 mL) in ethanol (20 mL) was subjected to hydrogenation at atmospheric pressure in the presence of 10% Pd/C (80 mg) for 4 h. The reaction mixture was filtered through Celite. The filtrate was concentrated to yield the hydrochloric salt of 4-hydroxybenzylamine as light yellow solids, which were used for the next step without further purification.

A mixture of 4-hydroxybenzylamine (395 mg, as the hydrochloric salt), 6-chloropurine riboside (143 mg, 0.5 mmol), and diisopropylethylamine (DIEA, 2 mL, 12 mmol) in 1-propanol (25 mL) was heated to 70° C. for 6 h. The mixture was concentrated under reduced pressure, and triturated with water to give white precipitates, which were filtered to yield the title compound 1 (151 mg, 81%). The purity of product was >99% as shown by HPLC on an Inertsil ODS-3 column (4.6×250 mm, 5 μm) with elution of 0.1% TFA/MeOH (6:4). C₁₇H₁₉N₅O₅; white powder, mp 208.7-209.2° C. (lit.⁹ mp 216-219° C.); [α]²⁰ _(D)=−64.5 (DMSO, c=1) (lit.⁹ [α]²⁵ _(D)=−87 (MeOH, c=0.1)); TLC (MeOH/EtOAc (1:9)) R_(f)=0.3; ¹H NMR (DMSO-d₆, 400 MHz) δ 9.22 (1H, s), 8.34 (1H, s), 8.30 (1H, br s), 8.18 (1H, s), 7.12 (2H, d, J=8.0 Hz), 6.65 (2H, d, J=8.0 Hz), 5.86 (1H, d, J=5.6 Hz), 5.41 (2H, m), 5.18 (1H, d, J=5.6 Hz), 4.60 (2H, m), 4.13 (1H, q, J=4.6, 7.4 Hz), 3.95 (1H, q, J=3.4, 6.2 Hz), 3.66 (1H, m), 3.53 (1H, m); ¹³C NMR (DMSO-d₆, 400 MHz) δ 155.3, 153.6, 151.6, 147.6, 139.1, 129.5, 127.9 (2×), 119.2, 114.4 (2×), 87.6, 85.6, 73.3, 70.5, 61.5, 42.4; ESI-HRMS calcd for C₁₇H₂₀N₅O₅: 374.1459, found: m/z 374.1412 [M+H]⁺.

N⁶-(4-Bromobenzyl)adenosine (2)

By a procedure similar to that for compound 1, 6-chloropurine riboside (98 mg, 0.34 mmol) was treated with 4-bromobenzylamine hydrochloride (5 equiv) and diisopropylethylamine (2 mL, 12 mmol) in 1-propanol (20 mL) at 70° C. for 7 h to give the title compound 2 (41 mg, 28%) after recrystallization from MeOH. The purity of product was 95% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN in 20 min. C₁₇H₁₉BrN₅O₄; white powder; mp 179.2-179.4° C.; [α]²⁶ _(D)=−50.1 (CH₃OH, c=1.0); TLC (MeOH/EtOAc (1:9)) R_(f)=0.69; IR ν_(max) (neat) 3333, 2925, 2865, 2358, 1621, 1486, 1408, 1336, 1298, 1224, 1121, 1081, 1011 cm⁻¹; ¹H NMR (DMSO-d₆, 400 MHz) δ 8.47 (1H, br s), 8.37 (1H, s), 8.19 (1H, s), 7.47 (2H, d, J=8.0 Hz), 7.28 (2H, d, J=8.0 Hz), 5.88 (1H, d, J=6.4 Hz), 5.43 (1H, d, J=6.0 Hz), 5.37 (1H, dd, J=2.4, 7.2 Hz), 5.18 (1H, d, J=4.8 Hz), 4.66 (2H, br s), 4.61 (1H, dd, J=5.6, 6.0 Hz), 4.08-4.16 (2H, m) 3.96 (1H, d, J=2.8 Hz), 3.64-3.69 (2H, m), 3.57-3.52 (2H, m); ¹³C NMR (DMSO-d₅, 100 MHz) δ 154.2, 152.1, 148.3, 139.9, 139.3, 130.9 (2×), 130.6 (2×), 119.6, 87.9, 85.9, 73.5, 70.7, 61.7, 42.4; ESI-HRMS calcd for C₁₇H₁₈BrN₅O₄: 436.0620, found: m/z 436.0654 [M+H]⁺.

N⁶-(4-Methoxybenzyl)adenosine (3)

By a procedure similar to that for 1, 6-chloropurine riboside (100 mg, 0.35 mmol) was treated with 4-methoxybenzylamine (243 mg, 1.77 mmol) and diisopropylethylamine (2 mL, 12 mmol) in 1-propanol (20 mL) at 70° C. for 7 h to give the title compound 3 (95 mg, 76%) after recrystallization from MeOH. The purity of product was >99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN in 20 min. C₁₆H₁₈N₆O₄; white powder; mp 147.6-148.2° C.; [α]²⁰ _(D)=−55.3 (DMSO, c=1.0) (lit.²¹ [α]²⁵ _(D)=−66.15 (MeOH, c=0.12)); TLC (MeOH/EtOAc (1:9)) R_(f)=0.60; ¹H NMR (DMSO-d₆, 400 MHz) δ 8.38 (2H, m), 8.19 (1H, br s), 8.30 (1H, br s), 7.25 (2H, d, J=8.4 Hz), 6.83 (2H, d, J=8.4 Hz), 5.87 (1H, d, J=6.0 Hz), 5.45-5.38 (2H, m), 5.19 (1H, d, J=3.2 Hz), 4.61 (2H, s), 4.14 (1H, s), 3.95 (1H, dd, J=3.2, 6.8 Hz), 3.70-3.64 (4H, m), 3.57-3.52 (1 H, m); ¹³C NMR (DMSO-d₆, 400 MHz) δ 157.8, 154.2, 152.1, 148.2, 139.7, 131.7, 128.3 (2×), 119.6, 113.4 (2×), 87.8, 85.8, 73.4, 70.6, 61.6, 55.0; ESI-MS calcd for C₁₆H₁₉N₆O₄: 388.1615, found: m/z 388.1620 [M+H]⁺.

N⁶-(3-Hydroxybenzyl)adenosine (4)

By a procedure similar to that for 1, 6-chloropurine riboside (143 mg, 0.5 mmol) was treated with 3-hydroxybenzylamine hydrochloric salt (395 mg, 2.46 mmol) and diisopropylethylamine (2 mL, 12 mmol) in 1-propanol (25 mL) at 70° C. for 6 h. The mixture was concentrated under reduced pressure, and triturated with water to give white precipitates, which were filtered to yield the title compound 4 (151 mg, 81%). The purity of product was >99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN in 20 min. C₁₇H₁₉N₅O₅; white powder, mp 195.6-196.5° C.; TLC (MeOH/EtOAc (1:9)) R_(f)=0.3; [α]²⁰ _(D)=−46.5 (DMSO, c=1.0); ¹H NMR (DMSO-d₆, 400 MHz) δ 9.22 (1H, s), 8.34 (1H, s), 8.30 (1H, br s), 8.18 (1 H, s), 7.12 (2H, d, J=8.0 Hz), 6.65 (2H, d, J=8.0 Hz), 5.86 (1H, d, J=5.6 Hz), 5.41 (2H, m), 5.18 (1H, d, J=5.6 Hz), 4.60 (2H, m), 4.13 (1H, q, J=4.6, 7.4 Hz), 3.95 (1 H, q, J=3.4, 6.2 Hz), 3.66 (1H, m), 3.53 (1H, m); ¹³C NMR (DMSO-d₆, 400 MHz) δ 155.3, 153.6, 151.6, 147.6, 139.1, 129.5, 127.9 (2×), 119.2, 114.4 (2×), 87.6, 85.6, 73.3, 70.5, 61.5, 42.4; ESI-HRMS calcd for C₁₇H₂₀N₅O₅: 374.1459, found: m/z 374.1412 [M+H]⁺.

N⁶-(4-Nitrobenzyl)adenosine (5)

By a procedure similar to that for 1, 6-chloropurine riboside (102 mg, 0.36 mmol) was treated with 4-nitrobenzylamine hydrochloride (336 mg, 1.78 mmol) and diisopropylethylamine (2 mL, 12 mmol) in 1-propanol (20 mL) at 70° C. for 7 h to give the title compound 5 (55.6 mg, 38%) after recrystallization from MeOH. The purity of product was >99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN in 20 min. C₁₇H₁₈N₆O₆; white crystals from MeOH; mp 167.8-168.6° C. (lit.⁴ mp 174-176° C.); [α]²⁰ _(D)=−50.7 (DMSO, c=0.5); TLC (MeOH/EtOAc (1:9)) R_(f)=0.45; ¹H NMR (DMSO-d₆, 400 MHz) δ 8.62 (1H, br s), 8.40 (1H, s), 8.18-8.14 (3H, m), 7.56 (2H, d, J=8.4 Hz), 5.88 (1H, d, J=6.0 Hz), 5.44 (1H, d, J=5.6 Hz), 5.34 (1H, t, J=5.6 Hz) 7.12 (2H, d, J=8.0 Hz); ¹³C NMR (DMSO-d₆, 400 MHz) δ 152.1, 147.9, 146.1, 140.0, 127.8 (2×), 123.3 (2×), 87.8, 85.8, 73.4, 70.6, 61.6, 42.7; EST-FIRMS calcd for C₁₇H₁₉N₆O₆: 403.1361, found: m/z 403.1372 [M+H]⁺.

N⁶-(3-Indolylethyl)adenosine (6)

In a round-bottomed flask (10 mL) were placed a solution of 6-chloropurine ribonucleoside (71 mg, 0.25 mmol), tryptamine (101 mg, 0.63 mmol) and diisopropylethylamine (0.24 mL, 2.88 mmol) in EtOH (3 mL). The flask was placed into the cavity of a focused monomode microwave reactor, and irradiated at 150 W for 10 min in refluxing EtOH. The mixture was concentrated by rotary evaporation, and the residue was purified by flash chromatography (silica gel; MeOH/EtOAc (1:9)) to give the title compound 6 (85 mg, 83%). The purity of product was 99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₀N₂₂N₆O₄; white powder; mp 187.0-187.2° C.; [α]²⁰ _(D)=−55.7 (CH₃OH, c=1.0); TLC (MeOH/EtOAc (1:9)) R_(f)=0.41; ¹H NMR (DMSO-d₆, 400 MHz) δ 10.78 (1H, s), 8.33 (1H, s), 8.25 (1 H, s), 7.96 (1H, br s), 7.61 (1H, d, J=7.2 Hz), 7.32 (1H, d, J=9.2 Hz), 7.18 (1H, s), 7.05 (1H, t, J=8.0 Hz), 5.80 (1H, d, J=6.0 Hz), 5.47-5.44 (2H, m), 5.20 (1H, d, J=4.4 Hz), 4.61 (1H, d, J=5.6 Hz), 4.14 (1H, d, J=2.8 Hz), 3.96 (1H, d, J=3.2 Hz), 3.77 (1H, br s), 3.69-3.52 (2H, m), 3.01 (2H, t, J=7.2 Hz); ¹³C NMR (DMSO-d₆, 100 MHz) δ 154.3, 152.2, 148.0, 139.5, 136.0, 127.1, 122.4, 120.8, 119.6, 118.3, 118.1, 111.7, 111.2, 87.9, 85.8, 73.4, 70.6, 61.6, 40.5, 25.1; ESI-HRMS calcd for C₂₀H₂₃N₆O₄: 411.1775, found: m/z 411.1750 [M+H]⁺.

5′-Chloro-5′-deoxy-N⁶-(4-methoxybenzyl)adenosine (7)

The reaction of 3-acetonide (2.96 g, 6.9 mmol) with p-toluenesulfonyl chloride (6.3 g, 34.6 mmol) in pyridine (6.0 mL) gave a mixture of sulfonate and the chloride derivatives (5:1) as shown by the ¹H NMR spectrum. The mixture was separated by flash chromatography (silica gel; CH₂Cl₂/MeOH (100:1)). The sulfonate compound showed ¹H NMR (CDCl₃, 400 MHz) δ 8.25 (1H, s), 7.66 (1H, s), 7.60 (2H, d, J=8.0 Hz), 7.29 (2H, d, J=8.4 Hz), 7.14 (2H, d, J=8.0 Hz), 6.86 (2H, d, J=8.4 Hz), 6.00 (1H, s), 5.31 (1H, d, J=6.0 Hz), 5.04 (1H, dd, J=3.2, 6.0 Hz), 4.78 (br, s), 4.43-4.47 (1H, m), 4.20-4.30 (2H, m), 3.78 (3H, s), 2.36 (3H, s), 1.58 (3H, s), 1.36 (3H, s). The sulfonate compound was unstable, and further conversion was not pursued.

The chloride compound (7-acetonide) showed ¹H NMR (CDCl₃, 400 MHz) δ 8.38 (1 H, s), 7.74 (1H, s), 7.29 (2H, d, J=8.4 Hz), 6.86 (2H, d, J=8.4 Hz), 6.07 (1H, s), 5.45 (1H, d, J=6.4 Hz), 5.16 (1H, s), 4.78 (br, s), 4.50 (1H, d, J=6.0 Hz), 3.83-3.77 (4H, m), 3.63-3.59 (1H, m), 1.62 (3H, s), 1.40 (3H, s). Compound 7-acetonide (8.5 mg, 0.019 mmol) was dissolved in 3 M HCl/THF (1:1, 130 μL). The mixture was stirred at room temperature for 14 h, and neutralized with saturated NaHCO₃ aqueous solution. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give the title compound 7 (5.9 mg, 70%). The purity of product was >99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₁₈H₂₀ClN₅O₄; white solid; mp 182.0-182.6° C.; TLC (EtOAc) R_(f)=0.4; [α]²¹ _(D)=−45.4 (THF, c=0.68); IR_(νmax) (neat) 3329, 3127, 1630, 1586, 1515, 1487, 1421, 1379, 1354, 1297, 1253, 1174, 1111, 1063, 1031 cm⁻¹; ¹H NMR (CD₃OD, 400 MHz) δ 8.26 (1H, s), 8.23 (1H, s), 7.30 (2H, d, 8.8 Hz), 6.86 (2H, d, J=8.8 Hz), 6.03 (1H, d, J=4.8 Hz), 4.77 (1H, t, J=5.2 Hz), 4.72 (2H, br s), 4.38 (1H, t, J=5.2 Hz), 4.27 (1H, t, J=4.8 Hz), 3.94 (1H, dd, J=6.8, 4.8 Hz), 3.83 (1H, dd, J=6.8, 4.8 Hz), 3.76 (1H, s); ¹³C NMR (DMSO-d₆, 100 MHz) δ 157.9, 154.2, 152.5, 148.6, 139.6, 131.8, 128.4 (2×), 119.4, 113.5 (2×), 87.5, 83.7, 72.7, 71.3, 55.1, 44.9, 42.4; ESI-HRMS calcd for C₁₈H₂₁ClN₅O₄: 406.1282, found: m/z 406.1279 [M+H]⁺.

5′-Azido-5′-deoxy-N⁶-(4-methoxybenzyl)adenosine (8)

The azido compound 8-acetonide (41.5 mg, 0.092 mmol) was stirred in 3 M HCl/THF (1:1, 0.26 mL) at room temperature for 14 h, and then neutralized with saturated NaHCO₃ aqueous solution. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give a quantitative yield of the title compound 8. The purity of product was 98% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₁₈H₂₀N₈O₄; white solid; mp 190.6-191.1° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.5; [α]²⁴ _(D)=−13.9 (CHCl₃, c=1.0); IR_(νmax) (neat) 3333, 2925, 2853, 2102, 1620, 1512, 1465, 1337, 1295, 1246, 1176, 1110, 1069, 1034 cm⁻¹; ¹H NMR (CD₃OD, 400 MHz) δ 8.27 (1H, br s), 8.24 (1H, br s), 7.30 (2H, d, J=8.4 Hz), 6.86 (2H, d, J=8.4 Hz), 6.02 (1H, d, 4.8 Hz), 4.77 (1 H, t, J=5.2 Hz), 4.73 (2H, br s), 4.36 (1H, t, J=5.2 Hz), 4.18 (1H, q, J=4.0 Hz), 3.65 (2H, t, J=3.6 Hz); ¹³C NMR (DMSO-d₆, 100 MHz) 157.9, 154.2, 152.4, 148.7, 139.7, 131.8, 128.4 (2×), 119.5, 113.5 (2×), 87.8, 72.8, 70.9, 55.1, 51.8, 42.4; ESI-HRMS calcd for C₁₈H₂₀N₈O₄: 413.1686, found: m/z 413.1686 [M+H]⁺.

5′-Azido-5′-deoxy-2′,3′-O-isopropylidene-N⁶-(4-methoxybenzyl)adenosine (8-acetonide)

To the acetonide of N⁶-(4-methoxybenzyl)adenosine (3-acetonide, 2.96 g, 6.9 mmol) in anhydrous pyridine (36 mL) was added a solution of p-toluenesulfonyl chloride (6.3 g, 34.6 mmol) in anhydrous pyridine (6.0 mL) dropwise via syringe to a solution of. The mixture was stirred at room temperature for 6 h. Pyridine was removed under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give a mixture of sultanate and the chloride derivatives (5:1) as shown by the ¹H NMR spectrum.

The above-prepared mixture was dissolved in anhydrous DMF (70 mL), and sodium azide (1.34 g, 20.6 mmol) was added. The mixture was stirred at 80° C. for 6 h, and then concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give a pale yellow oil, which was purified by flash chromatography (silica gel; CH₂Cl₂/MeOH (100:1)) to give 8-acetonide (653 mg, 21% overall yield). C₂₁H₂₄N₈O₄; colorless oil; TLC (EtOAc/Hexane (6:4)) R_(f)=0.39; [α]²³ _(D)=+5.0 (EtOAc, c=1.0); IR ν_(max) (neat) 3280, 2987, 2931, 2101, 1618, 1512, 1478, 1375, 1330, 1296, 1248, 1211, 1154, 1091 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.38 (1H, br s), 7.72 (1H, br s), 7.26 (2H, d, J=8.8 Hz), 6.84 (2H, d, J=8.8 Hz), 6.37 (1H, br s), 6.06 (1H, d, J=2.0 Hz), 5.46-5.44 (1H, m), 5.07-5.05 (1H, m), 4.77 (2H, br s), 4.38-4.35 (1H, m), 3.77 (3H, s), 3.51-3.62 (2H, m), 1.61 (3H, s), 1.39 (3H, s); ¹³C NMR (CDCl₃, 100 MHz) δ 158.7, 154.5, 153.1, 147.9, 139.0, 130.2, 128.8 (2×), 120.1, 114.4, 113.8 (2×), 90.4, 85.6, 83.9, 82.0, 55.2, 52.2, 43.7, 29.7, 27.1, 25.3; ESI-HRMS calcd for C₂₁H₂₄N₈O₄: 453.1999, found: m/z 453.1999 [M+H]⁺.

5′-Acetamido-5′-deoxy-N⁶-(4-methoxybenzyl)adenosine (9)

The azido compound 8-acetonide (95 mg, 0.21 mmol) was stirred with triphenylphosphine (66 mg, 0.24 mmol) in THF/H₂O (10:1, 2 mL) at room temperature for 4.5 h. The mixture was concentrated under reduced pressure. The residue was taken up with CH₂Cl₂ and H₂O, and acidified with HCl solution (1 M) until pH=2. The aqueous phase was separated, neutralized with saturated NaHCO₃ aqueous solution, and extracted with CH₂Cl₂. The organic extract was dried over MgSO₄, filtered, and concentrated to yield a crude amine product.

The crude amine was treated with acetic anhydride (98.6 μL, 1.05 mmol) in anhydrous pyridine (0.2 mL). The mixture was stirred at room temperature for 1.5 h, and then concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, concentrated, and purified by flash chromatography (silica gel; CH₂Cl₂/MeOH (98:2)) to give the acetonide of compound 9 (56 mg, 57% yield for 2 steps). The purity of product was >99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₃H₂₈N₆O₅; colorless oil; TLC (CH₂Cl₂/MeOH (98:2)) R_(f)=0.2; [α]²⁵ _(D)=−146.6 (CHCl₃, C=1.0); IR ν_(max) (neat) 3280, 3062, 2989, 2930, 2835, 2358, 1667, 1620, 1513, 1376, 1336, 1296, 1246, 1215, 1096, 1034 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.36-8.38 (2H, m), 7.73 (1H, s), 7.28 (2H, d, J=8.8 Hz), 6.86 (2H, d, J=8.8 Hz), 6.15 (1H, br s), 5.77 (1H, d, J=4.8 Hz), 5.26 (1H, t, J=4.8 Hz), 4.81 (1H, dd, J=4.0, 2.4 Hz), 4.76 (2H, br s), 4.47-4.48 (1H, m), 4.11-4.17 (1H, m), 3.79 (3H, s), 3.24 (1H, d, J=14.4 Hz), 2.15 (3H, s), 1.61 (3H, s), 1.34 (3H, s); ¹³C NMR (CDCl₂, 100 MHz) δ 170.5, 158.8, 154.8, 152.7, 147.7, 139.7, 130.0, 128.9 (2×), 121.1, 114.6, 113.9 (2×), 92.5, 83.3, 82.2, 81.3, 55.3, 43.9, 41.1, 27.6, 25.4, 23.2; ESI-HRMS calcd. for C₂₃H₂₈N₆O₅: 469.2190, found m/z 469.2193 [M+H]⁺.

The acetonide of 9 (17.2 mg, 0.037 mmol) was stirred in 3 M HCl/THF (1:1, 0.1 mL) at room temperature for 14 h, and then neutralized with saturated NaHCO₃ aqueous solution. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give the title compound 9 (11 mg, 70%). The purity of product 9 was 99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN in 20 min. C₂₀H₂₄N₆O₅; white powder; mp 121.1-121.6° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.5; [α]²⁵ _(D)=−108.7 (THF, c=0.89); IR ν_(max) (neat) 3275, 3071, 2923, 2852, 2360, 1621, 1512, 1375, 1339, 1297, 1245, 1175, 1126, 1076 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.76 (1H, s), 8.27 (1H, s), 7.24 (2H, d, J=8.4 Hz), 6.81 (2H, d, J=8.4 Hz), 6.54 (1H, s), 5.70 (1H, d, J=5.6 Hz), 4.72 (3H, d, J=5.6 Hz), 4.23 (1H, s), 4.18 (1H, s), 3.98-4.03 (1H, m), 3.75 (3H, s), 3.13 (1 H, d, J=14.0 Hz), 2.02 (3H, s); ¹³C NMR (DMSO, 100 MHz) 169.4, 157.9, 154.3, 152.3, 148.3, 140.2, 131.8, 128.4 (2×), 119.8, 113.5 (2×), 87.9, 83.6, 72.6, 71.3, 55.1, 42.4, 41.1, 22.7; ESI-HRMS calcd for C₂₀H₂₄N₆O₅: 427.1730, found: m/z 427.1727 [M+H]⁺.

5′-(p-Tolylsulfonamido)-5′-deoxy-N⁶-(4-methoxybenzyl)adenosine (10)

The crude amine compound (˜72.4 mg), prepared by Staudinger reduction of 8-acetonide, was dissolved in anhydrous pyridine (1.1 mL), and a solution of p-toluenesulfonyl chloride (159.4 mg, 0.87 mmol) in anhydrous pyridine (1.12 mL) was added dropwise via syringe. The mixture was stirred at room temperature for 4.5 h. Pyridine was removed under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give a colorless oil, which was purified by flash chromatography (silica gel; CH₂Cl₂/MeOH (120:1 to 100:1)) to give the acetonide of compound 10 (40.4 mg, 41% yield). C₂₈H₃₂N₆O₆; TLC (EtOAc/Hexane (10:1)) R_(f)=0.25; [α]²⁷ _(D)=+2.7 (CHCl₃, c=1.0); IR_(νmax) (neat) 2925, 2862, 2360, 1621, 1513, 1480, 1383, 1329, 1301, 1247, 1215, 1158, 1087, 1034 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 9.29 (1H, d, J=10.0 Hz), 8.60 (1H, s), 7.71 (2H, d, J=8.0 Hz), 7.63 (1H, s), 7.28 (2H, d, J=8.4 Hz), 7.24 (2H, d, J=8.0 Hz), 6.85 (2H, d, J=8.4 Hz), 6.35 (1H, br s), 5.70 (1H, d, J=4.8 Hz), 5.24 (1H, t, J=5.6 Hz), 4.93 (1H, d, J=6.0 Hz), 4.78 (2H, br s), 4.49 (1H, d, J=1.2 Hz), 3.79 (3H, s), 3.43-3.47 (1H, m), 3.13 (1H, dd, J=2.4, 10.8 Hz), 2.37 (3H, s), 1.59 (3H, s), 1.31 (3H, s); ¹³C NMR (CDCl₃, 100 MHz) δ 158.9, 154.8, 153.1, 142.8, 139.2, 137.2, 130.0, 129.6 (2×), 129.0 (2×), 126.7 (2×), 114.5, 114.0 (2×), 93.4, 82.8, 82.3, 82.6, 55.3, 45.3, 44.1, 27.6, 25.2, 21.5; ESI-HRMS calcd for C₂₈H₃₂N₆O₆S: 581.2182, found: m/z 581.2198 [M+H]⁺.

The acetonide of 10 (41.7 mg, 0.072 mmol) was stirred in 3 M HCl/THF (1:1, 0.2 mL) at room temperature for 14 h, and then neutralized with saturated NaHCO₃ aqueous solution. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give the crude product, which was purified by flash chromatography (silica gel; CH₂Cl₂/MeOH=25:1) to give the title compound 10 (68%). The purity of product was >99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₅H₂₇N₆O₆S; TLC (EtOAc/Hexane (10:1)) R_(f)=0.25; [α]²⁶ _(D)=+25.9 (THF, c=0.8); IR ν_(max) (neat) 3356, 2926, 2860, 2362, 1620, 1513, 1461, 1330, 1301, 1247, 1159, 1069, 1038 cm⁻¹; ¹H NMR (CD₃OD, 400 MHz) δ 7.91 (1H, s), 7.66 (1H, s), 7.27 (2 H, d, J=8.0 Hz), 6.84-6.87 (4H, m), 6.41 (2H, d, J=8.4 Hz), 5.40 (1H, d, J=6.4 Hz), 4.28 (3H, br s), 3.77-3.82 (3H, m), 2.88-2.89 (1H, m), 2.85 (1H, d, J=2.8 Hz), 2.71 (1H, d, J=2.8 Hz), 2.68 (1H, d, J=3.2 Hz), 1.92 (3H, s); ¹³C NMR (CD₃OD, 100 MHz) δ 160.6, 156.3, 153.9, 149.3, 144.9, 142.0, 138.7, 132.2, 130.9 (2×), 130.1 (2×), 128.0 (2×), 121.7, 115.1 (2×), 91.6, 85.9, 74.4, 73.1, 55.9, 46.2, 44.8, 21.6; ESI-HRMS calcd for C₂₅H₂₈N₆O₆S: 541.1869, found: m/z 541.1877 [M+H]⁺.

5′-Deoxy-5′-(4-methyl-1,2,3-triazol-1-yl)-N⁶-(4-methoxybenzyl)adenosine (11)

A mixture of azido compound 8-acetonide (313 mg, 0.69 mmol), CuSO₄.5H₂O (24.9 mg), sodium ascorbate (61.4 mg) and propargyl alcohol in H₂O/t-BuOH (1:1, 7 mL) was stirred at room temperature for 12 h, and then concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to yield a triazole acetonide (˜220 mg) as colorless oil. TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.5; ESI-HRMS calcd for C₂₄H₂₈N₈O₅: 509.2261, found: m/z 509.2267 [M+H]⁺.

The above-prepared triazole compound was stirred with triethylamine (0.15 mL, 1.08 mmol) and methylsulfonyl chloride (0.08 mL, 1.08 mmol) in anhydrous CH₂Cl₂ (4.3 mL) at room temperature for 2 h. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to yield a mesylate compound as colorless oil. TLC (EtOAc/Hex (4:1)) R_(f)=0.45; ESI-HRMS calcd for C₂₅H₃₀N₈O₇SNa: 609.1856, found: m/z 609.1876 [M+Na]⁺.

The mesylate was treated with NaBH₄ (24.5 mg, 0.65 mmol) at 0° C. in DMF, and then heated to 60° C. for 6 h. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give 11-acetonide as colorless oil. TLC (EtOAc/Hex (4:1)) R_(f)=0.25; ESI-HRMS calcd for C₂₄H₂₈N₈O₄: 493.2312, found: m/z 493.2312 [M+H]⁺. The acetonide of 11 was stirred in 3 M HCl/THF (1:1, 0.33 mL) at room temperature for 14 h, and then neutralized with saturated NaHCO₃ aqueous solution. The mixture was concentrated under reduced pressure, and the residue was dissolved in THF, filtered, and concentrated to give the title compound 11 (48.1 mg, 25% overall yield). The purity of product was 98% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₁H₂₄N₈O₄; white powder; mp 183.0-183.2° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.12; [α]²⁷ _(D)=+20.3 (CH₃OH, c=0.45); IR ν_(max) (neat) 3217, 2921, 2850, 2685, 1620, 1513, 1470, 1337, 1297, 1244, 1176, 1111, 1058 cm⁻¹; ¹H NMR (CD₃OD, 400 MHz) δ 8.22 (1H, s), 7.99 (1H, s), 7.45 (1H, s), 7.31 (2H, d, J=8.8 Hz), 6.87 (2H, d, J=8.8 Hz), 5.96 (1H, d, J=4.0 Hz), 4.82-4.68 (5H, m), 4.46 (1H, t, J=4.0 Hz), 4.34 (1H, q, J=4.0 Hz), 3.77 (3H, s), 2.15 (3H, s); ¹³C NMR (CDCl₃, 100 MHz) δ 158.7, 154.2, 152.9, 148.0, 142.8, 138.8, 130.1, 128.8 (2×), 123.1, 119.6, 113.8 (2×), 89.3, 82.2, 73.4, 70.8, 55.2, 50.9, 43.9, 10.5; ESI-HRMS (negative mode) calcd for C₂₁H₂₄N₈O₄: 451.1842, found: m/z 451.1843 [M−H]⁻.

5′-(4-Butyl-1,2,3-triazol-1-yl)-5′-deoxy-N⁶-(4-methoxybenzyl)adenosine (12)

A mixture of azido compound 8 (13.31 mg, 0.032 mmol), CuSO₄.5H₂O (0.40 mg), sodium ascorbate (0.95 mg) and 1-hexyne in H₂O/t-BuOH (1:1, 430 μL) was stirred at room temperature for 30 h, and then concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give the title compound 12 (12.82 mg, 81%). The purity of product was >95% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₄H₃₀N₈O₄; white powder; mp 105.0-105.4° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.38; [α]²³ _(D)=−12.2 (CHCl₃, c=1.0); IR ν_(max) (neat) 3333, 3140, 2929, 2858, 2360, 1619, 1512, 1464, 1333, 1297, 1245, 1176, 1094, 1037 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.28 (1H, s), 7.73 (1H, s), 7.27 (2H, d, J=8.4 Hz), 7.15 (1H, s), 6.84 (2H, d, J=8.4 Hz), 6.13 (1H, br s), 5.89 (1H, d, 5.2 Hz), 4.73 (3H, dd, J=9.6, 4.8 Hz), 4.61-4.67 (2H, m), 4.51 (1H, d, J=4.4 Hz), 4.34 (1H, br s), 3.78 (3H, s), 2.45-2.57 (2H, m), 1.39-1.46 (2H, m), 1.19-1.28 (2H, m), 0.84 (3H, t, J=7.6 Hz); ¹³C NMR (CDCl₃, 100 MHz) δ 158.8, 154.3, 152.9, 148.5, 148.0, 138.8, 130.1, 128.9 (2×), 122.5, 119.7, 113.9 (2×), 89.5, 82.5, 73.5, 70.9, 55.3, 51.1, 44.0, 31.2, 25.0, 22.3, 13.8; ESI-HRMS calcd for C₂₄H₃₀N₈O₄: 493.2312, found: m/z 493.2316 [M+H]⁻.

5′-Deoxy-5′-(4-hexyl-1,2,3-triazol-1-yl)-N⁶-(4-methoxybenzyl)adenosine (13)

A mixture of azido compound 8 (10.30 mg, 0.025 mmol), CuSO₄.5H₂O (0.31 mg), sodium ascorbate (0.73 mg) and 1-octyne (4.46 μL, 0.03 mmol) in H₂O/t-BuOH (1:1, 333 μL) was stirred at room temperature for 37 h, and then concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give the title compound 13 (12.36 mg, 94.6%). The purity of product was >98% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₆H₃₄N₈O₄; white powder; mp 101.4-102.1° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.33; [α]²⁵ _(D)=−9.7 (CHCl₃, c=1.0); IR σ_(max) (neat) 3332, 3148, 2926, 2855, 2359, 1620, 1513, 1463, 1336, 1297, 1246, 1175, 1109, 1037 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.26 (1H, s), 7.67 (1H, s), 7.26 (2H, d, J=8.4 Hz), 7.16 (1H, s), 6.82 (2H, d, J=8.4 Hz), 6.31 (1H, br s), 5.91 (1H, d, J=4.4 Hz), 4.63-4.75 (4H, m), 4.58 (1H, t, J=4.8 Hz), 4.44-4.51 (2H, m), 3.76 (3H, s), 2.42-2.56 (2H, m), 1.20-1.24 (8H, m), 0.82 (3H, t, J=6.8 Hz); ¹³C NMR (DMSO-d₆, 100 MHz) δ 157.9, 154.2, 152.5, 148.4, 146.7, 139.7, 131.8, 128.4 (2×), 122.5, 119.5, 113.6 (2×), 93.9, 87.9, 82.4, 72.7, 70.9, 55.1, 51.2, 42.4, 31.1, 28.9, 28.3, 25.0, 22.2, 14.1; ESI-HRMS calcd for C₂₆H₃₄N₈O₄: 521.2625, found: m/z 521.2628 [M+H]⁻.

5′-(4-Benzyl-1,2,3-triazol-1-yl)-5′-deoxy-N⁶-(4-methoxybenzyl)adenosine (14)

A mixture of azido compound 8 (11.97 mg, 0.029 mmol), CuSO₄.5H₂O (0.36 mg), sodium ascorbate (0.85 mg) and 3-phenyl-1-propyne in H₂O/t-BuOH (1:1, 386 μL) was stirred at room temperature for 30 h, and then concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, and concentrated to give the title compound 14 (12.57 mg, 82%). The purity of product was 99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN. C₂₇H₂₈N₈O₄; white powder, mp 123.3-123.9° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.38; [α]²⁶ _(D)=+9.3 (CHCl₃, c=1.0); IR ν_(max) (neat) 3329, 3143, 3029, 2925, 2835, 2359, 1620, 1479, 1434, 1333, 1296, 1245, 1176, 1109, 1034 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.27 (1H, s), 7.66 (1H, s), 7.27 (2H, d, J=8.8 Hz), 7.10-7.19 (3H, m), 7.03-7.06 (3H, m), 6.81 (2H, d, J=8.8 Hz), 6.20 (1H, br s), 5.86 (1H, d, J=4.8 Hz), 4.75 (2H, br s), 4.67 (1H, dd, 10.0, 4.8 Hz), 4.56-4.60 (2H, m), 4.36 (1H, t, J=4.4 Hz), 3.86 (2H, dd, J=18.4, 16.0 Hz), 3.75 (3H, s); ¹³C NMR (CDCl₃, 100 MHz) 158.8, 154.2, 152.9, 147.1, 138.8, 138.2, 130.1, 128.9 (2×), 128.4 (2×), 128.3 (2×), 126.4, 123.3, 119.7, 113.9 (2×), 89.4, 82.3, 73.6, 70.9, 55.3, 51.1, 44.0, 31.8; ESI-HRMS calcd for C₂₇H₂₈N₈O₄: 527.2155, found: m/z 527.2140 [M+H]⁻.

3,4-Dihydroxy-5-[6-(4-hydroxybenzylamino)-purin-9-yl]-tetrahydrofuran-2-carboxylic acid ethylamide (15)

The acetonide derived from 6-chloropurine ribofuranoside (17-acetonide, 158 mg, 0.48 mmol) was stirred with PhI(OAC)₂ (509 mg, 1.56 mmol) and 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO, 15.4 mg, 0.1 mmol) in a degassed CH₃CN/H₂O solution (1:1, 2.6 mL) at 40° C. for 4 h. The mixture was concentrated under reduced pressure to yield a crude acid product 18.

The crude acid was treated with ethylamine (117 mg, as the hydrochloric salt), O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU, 375 mg, 0.72 mmol) and diisopropylethylamine (0.5 ml, 2.89 mmol) in anhydrous DMF (11.6 mL) at room temperature for 14 h. The mixture was concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, concentrated, and purified by flash chromatography (silica gel; EtOAc/hexane (1:1)) to yield an amide product 19 as colorless oil.

The amide product was treated with 4-hydroxybenzylamine (385 mg, 2.4 mmol as the hydrochloric salt) and diisopropylethylamine (2.8 mL, 16.9 mmol) in 1-propanol (28 mL) at 70° C. for 2 h. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, concentrated, and purified by flash chromatography (silica gel; CH₂Cl₂/MeOH (97:3)) to give 15-acetonide (179 mg, 82%). C₂₃H₂₈N₆O₅; colorless oil; TLC (EtOAc/hexane (4:1)) R_(f)=0.13; [α]²² _(D)=−32.0 (EtOAc, c=1.0); IR ν_(max) (neat) 3347, 3103, 2982, 2933, 1732, 1667, 1615, 1516, 1479, 1461, 1376, 1332, 1295, 1245, 1212, 1154, 1088 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 9.01 (1H, br s), 8.35 (1H, br s), 7.81 (1H, s), 7.14 (1H, br s), 7.04 (2H, d, J=8.0 Hz), 6.68 (2H, d, J=8.0 Hz), 6.49 (1H, t, 4.8 Hz), 6.03 (1H, d, J=2.4 Hz), 5.33-5.38 (2H, m), 4.70 (3H, s), 3.09-3.16 (2H, m), 1.62 (3H, s), 1.37 (3H, s), 0.90 (3H, t, J=7.2 Hz); ¹³C NMR (CDCl₃, 100 MHz) δ 168.7, 155.8, 154.2, 153.1, 147.7, 139.1, 128.8 (3×), 119.6, 115.4 (2×), 114.3, 91.6, 85.6, 83.3, 82.4, 43.9, 34.0, 27.0, 25.1, 14.2; ESI-HRMS calcd for C₂₂H₂₆N₆O₅: 455.2043, found: m/z 455.2037 [M+H]⁺.

The acetonide of 15 (26 mg, 0.057 mmol) was stirred in 1 M HCl/THF (1:1, 0.3 mL) at room temperature for 16 h, and then neutralized with saturated NaHCO₃ aqueous solution. After concentration, the residue was triturated with H₂O to give the title compound 15, which was then recrystallized from MeOH (14.65 mg, 62%). C₁₉H₂₂N₆O₅; white powder, mp 179.7-180.5° C.; TLC (EtOAc) R_(f)=0.04; [α]²³ _(D)=−27.7 (MeOH, c=1.0); IR ν_(max) (neat) 3256, 2688, 2360, 1618, 1515, 1335, 1294, 1232, 1128, 1052 cm⁻¹; ¹H NMR (CD₃OD, 400 MHz) 8.29 (1H, s), 8.22 (1H, s), 7.20 (2H, d, J=8.4 Hz), 6.73 (2H, d, J=8.4 Hz), 6.00 (1H, d, J=7.6 Hz), 4.76-4.73 (1H, m), 4.70 (2H, br s), 4.46 (1H, s), 4.30-4.31 (1H, m), 3.36 (2H, q, 7.2 Hz), 1.21 (3H, t, 7.2 Hz); ¹³C NMR (CD₂OD, 100 MHz) δ 171.8, 157.5, 155.8, 153.6, 149.1, 141.9, 130.5, 129.9 (2×), 121.3, 116.2 (2×), 90.5, 86.3, 74.9, 73.4, 44.9, 35.2, 15.3; ESI-HRMS calcd for C₁₉H₂₁N₆O₅: 413.1573, found: m/z 413.1573 [M−H]⁺; Anal. Calcd for C₁₉H₂₂N₆O₅.H₂O: C, 52.77; H, 5.59; N, 19.43; found: C, 52.88; H, 5.40; N, 19.44.

2-[6-(4-Methoxybenzylamino)-purin-9-yl]-5-(1H-tetrazol-5-yl)-tetrahydrofuran-3,4-diol (16)

The crude acid 18 obtained from oxidation of 17-acetonide (ca. 3.98 mmol) with PhI(OAc)₂/TEMPO was treated with ammonium chloride (426 mg, 7.96 mmol), benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP, 3.07 g, 5.97 mmol), hydroxybenzotriazole (HOBt, 807 mg, 5.97 mmol) and diisopropylethylamine (2.5 mL, 15.9 mmol) in anhydrous DMF (40 mL) at 50° C. for 14 h. The mixture was concentrated under reduced pressure. The residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, concentrated, and purified by flash chromatography (silica gel; EtOAc/hexane (1:1 to 4:1)) to yield an amide product 20 as colorless oil.

A solution of dimethyl sulfoxide (0.85 mL, 11.9 mmol) in CH₂Cl₂ (10 mL) was added to a solution of oxalyl chloride (0.7 mL, 7.96 mmol) in CH₂Cl₂ (10 mL) at −78° C. The mixture was stirred for 30 min, and a solution of amide 20 (ca. 3.98 mmol) in CH₂Cl₂ (20 mL) was added. The mixture was stirred at −78° C. for another 30 min, and diisopropylethylamine (2.6 mL, 15.9 mmol) was added. After 1 h stirring, formation of nitrile 21 was monitored by TLC analysis. The mixture was extracted with CH₂Cl₂ and H₂O. The organic phase was dried over MgSO₄, filtered, concentrated, and purified by flash chromatography (silica gel; EtOAc/hexane (2:3)) to yield nitrile 21 as colorless oil (863 mg) contaminated with a small amount of HOBt.

The above-prepared nitrile product (863 mg, 2.68 mmol) was treated with 4-methoxybenzylamine (1.84 g, 13.4 mmol) and diisopropylethylamine (15.5 mL) in 1-propanol (26 mL) at 70° C. for 4 h. The mixture was concentrated under reduced pressure, and the residue was extracted with CH₂Cl₂ and H₂O. The organic layer was dried over MgSO₄, filtered, concentrated, and purified by flash chromatography (silica gel; CH₂Cl₂/MeOH (300:1 to 150:1)) to give compound 22 (905 mg, 54% overall yield). C₂₁H₂₂N₆O₄; colorless oil; TLC (EtOAc/hexane (4:1)) R_(f)=0.55; [α]²⁶ _(D)=+25.8 (EtOAc, c=1.0); IR ν_(max) (neat) 3373, 3282, 2990, 2925, 2853, 1679, 1618, 1512, 1465, 1376, 1331, 1295, 1249, 1212, 1135, 1086 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 8.39 (1H, br s), 7.64 (1H, br s), 7.26 (2H, d, J=10.4 Hz), 6.83 (2H, d, J=10.4 Hz), 6.54 (1H, t, J=5.6 Hz), 6.13 (1H, s), 5.77 (1H, d, J=4.0 Hz), 5.68 (1H, dd, J=1.6, 4.0 Hz), 4.95 (1H, d, J=1.6 Hz), 4.75 (2H, br s), 3.79 (3H, s), 1.57 (3H, s), 1.42 (3H, s); ¹³C NMR (CDCl₃, 100 MHz) δ 158.8, 154.5, 153.2, 148.2, 138.9, 130.2, 128.9 (2×), 119.7, 115.9, 114.5, 113.9 (2×), 91.6, 84.6, 83.9, 75.1, 55.3, 44.0, 26.6, 25.1; ESI-HRMS (negative mode) calcd for C₂₁H₂₂N₆O₄: 421.1624, found: m/z 421.1612 [M−H]⁻.

A solution of nitrile 22 (905 mg, 2.14 mmol) and NH₄Cl (429 mg, 8.04 mmol) in DMF (20 mL) was cooled to 0° C., and added NaN₃ (523 mg, 8.04 mmol). The ice bath was removed; the mixture was heated to 40° C. for 1 h, slowly to 90° C., and kept stirring at 90° C. for 9 h. The mixture was cooled, concentrated under reduced pressure, dissolved in EtOAc, and extracted with NaHCO₃ aqueous solution (pH=8). The combined aqueous phase was acidified by addition of HCl solution (1 M) until pH=2, and extracted with CH₂Cl₂. The organic layer was dried over MgSO₄, filtered, and concentrated to give a practically pure tetrazole product 16-acetonide as colorless oil (460 mg, 46% yield). C₂₁H₂₃N₉O₄; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.25; [α]²⁷ _(D)=+13.2 (EtOAc, c=1.0); IR ν_(max) (neat) 3361, 2926, 2852, 1613, 1513, 1481, 1375, 1333, 1293, 1249, 1210, 1176, 1154, 1101, 1034 cm⁻¹; ¹H NMR (CDCl₃, 400 MHz) δ 7.90 (1H, br s), 7.68 (1H, br s), 7.35 (2H, d, J=8.4 Hz), 6.86 (2H, d, J=8.4 Hz), 6.83 (1H, br s), 6.18 (1 H, s), 5.85 (1H, s), 5.73 (1H, d, J=6.0 Hz), 5.49 (1H, d, J=6.0 Hz), 4.92 (1H, dd, J=6.8, 7.6 Hz), 4.39 (1H, dd, J=4.0, 10.4 Hz), 3.77 (3H, s), 1.69 (3H, s), 1.43 (3H, s); ¹³C NMR (CDCl₃, 100 MHz) δ 158.4, 154.9, 152.9, 152.6, 146.2, 138.5, 129.5, 129.2 (2×), 118.4, 114.2, 113.7 (2×), 93.4, 85.9, 83.7, 82.3, 55.4, 44.1, 27.1, 25.2; ESI-HRMS (negative mode) calcd for C₂₁H₂₃N₉O₄: 464.1795, found: m/z 1786 [M−H]⁻.

Compound 16-acetonide (460 mg, 0.99 mmol) was stirred in 3 M HCl/THF (1:1, 0.1 mL) at room temperature for 14 h, and then neutralized with saturated NaHCO₃ aqueous solution. The mixture was concentrated under reduced pressure; the residue was taken up with THF, filtered, and concentrated to give the title compound 16 (320 mg, 76%). The purity of product was 99% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm) with elution of gradients of 30-60% aqueous CH₃CN in 20 min. C₁₈H₁₃N₉O₄; white powder; mp 210.0-210.6° C.; TLC (CH₂Cl₂/MeOH (9:1)) R_(f)=0.05; [α]²⁶ _(D)=−25.8 (THF, c=1.0); IR ν_(max) (neat) 3397, 2841, 2692, 1623, 1511, 1475, 1419, 1339, 1302, 1236, 1180, 1124, 1045 cm⁻¹; ¹H NMR (DMSO-d₆, 400 MHz) δ 8.82 (1H, s), 8.30 (1H, br s), 8.20 (1H, s), 7.26 (2H, d, J=8.0 Hz), 6.83 (2H, d, J=8.0 Hz), 6.08 (1H, d, J=5.6 Hz), 5.53 (1H, d, J=6.0 Hz), 5.46 (1H, d, J=2.8 Hz), 5.18 (1H, s), 4.91 (1H, d, J=5.2 Hz), 4.62 (2H, br s), 4.20 (1H, s), 3.69 (3H, d, J=2.0 Hz); ¹³C NMR (DMSO-d₆, 100 MHz) δ 159.9, 157.4, 153.7, 151.9, 138.8, 131.5, 127.9 (2×), 113.1 (2×), 85.9, 79.1, 75.4, 74.3, 54.6, 41.9; ESI-HRMS (negative mode) calcd for C₁₈H₁₉N₃O₄: 427.1730, found: m/z 427.1727 [M−H]⁻.

6-Chloro-9-(2′,3′-O-isopropylidene-β-D-ribofuranosyl)-purine (17-acetonide)

A mixture of 6-chloropurine riboside (286 mg, 1.0 mmol), 2,2-dimethoxypropane (0.6 mL, 4.90 mmol) and p-toluenesulfonic acid monohydrate (420 mg, 2.21 mmol) in anhydrous acetone (10 mL) heated at 55° C. for 2 h. The solution was poured slowly into a stirring saturated NaHCO₃ (10 mL), and extracted with CH₂Cl₂. The combined organic layer was dried over anhydrous MgSO₄, concentrated by rotary evaporation under reduced pressure, and purified by flash chromatography (silica gel; EtOAc/hexane (1:1)) to yield the title compound 17-acetonide (264 mg, 81%). C₁₃H₁₅ClN₄O₄; white powder; mp 157.4-158.8° C.; TLC (EtOAc/hexane (1:1)) R_(f)=0.23; ¹H NMR (CDCl₃, 400 MHz) δ 8.74 (1H, s), 8.26 (1H, s), 5.98 (1H, d, J=4.4 Hz), 5.17 (1H, t, J=5.2 Hz), 5.08 (1H, d, J=6.0 Hz), 4.98 (1H, br s), 4.53 (1H, s), 3.95 (1H, m), 3.81 (1H, m), 1.63 (3H, s), 1.36 (3H, s); ¹³C NMR (CDCl₃, 400 MHz) δ 151.7, 151.4, 150.2, 144.5, 132.8, 114.4, 94.0, 86.6, 83.7, 81.8, 63.5, 28.1, 25.8; ESI-HRMS calcd for C₁₃H₁₆ClN₄O₄: 327.0860, found: m/z 327.0913 [M+H]⁺.

N⁶-(4-Dimethylaminobenzyl)adenosine (23)

By a procedure similar to that for compound 1,6-chloropurine riboside (100 mg, 0.35 mmol) was treated with 4-dimethylaminobenzylamine hydrochloride (5 equiv) and diisopropylethylamine (2 mL, 12 mmol) in 1-propanol (20 mL) at 70° C. for 7 h to give the title compound 23 (68 mg, 49%) after recrystallization from MeOH. C₁₉H₂₄N₆O₄; white powder; mp 170.2-170.7° C.; [α]²⁰ _(D)=−64.5 (DMSO, 1=10 cm); TLC (MeOH/EtOAc (1:9)) R_(f)=0.58; ¹H NMR (DMSO-d₆, 400 MHz) δ 8.34 (1H, s), 8.28 (1H, br s), 8.19 (1H, br s), 7.15 (2H, d, J=8.8 Hz), 6.63 (2 d, J=8.8 Hz), 5.87 (1H, d, J=6.0 Hz), 5.44-5.40 (2H, m), 5.18 (1H, d, J=4.4 Hz), 4.62-4.58 (3H, m), 4.14 (1H, dd, J=4.4, 8.0 Hz), 3.95 (1H, d, J=4.4 Hz), 3.68-3.64 (1H, m), 3.57-3.54 (1H, m), 2.81 (6H, s); ¹³C NMR (DMSO-d₆, 400 MHz) δ 154.2, 152.1, 149.2, 148.1, 139.6, 128.0 (2×), 127.3, 119.6, 112.2 (2×), 87.9, 85.8, 73.4, 70.6, 61.6, 42.4, 40.3 (2×); ESI-MS calcd for C₁₉H₂₅N₆O₄: 4801.1932, found: m/z 401.1964 (M⁺+H).

N⁶-(1-Pyrenylmethyl)adenosine (24)

In a round bottom flask (10 mL) were added 6-chloropurine riboside (70 mg, 0.25 mmol) and 1-pyrenemethylamine hydrochloride (167 mg, 0.63 mmol) in EtOH (3 mL). Diisopropylethylamine (0.24 mL, 2.88 mmol) was added as the base. The round bottom flask was placed into the cavity of a focused monomode microwave reactor (CEM Discover) and irradiated in 150 W for 10 min at the boiling point of EtOH. The solvent was removed by rotavapor and the residue was purified by flash chromatography (silica gel; EtOAc/MeOH (95:5)) to yield the desired product 24 (59 mg, 49%). C₂₇H₂₃N₅O₄; yellow powder; mp 143.9-144.4° C.; [α]²⁰ _(D)=−50.4 (DMSO, c=1.0); TLC (EtOAc/MeOH (9:1)) R_(f)=0.43; ¹H NMR (DMSO-d₆, 400 MHz) δ 8.68 (1H, br s), 8.54 (1H, d, J=9.2 Hz), 8.39 (1H, s), 8.28 (2H, d, J=7.6 Hz), 8.25-8.19 (4H, m), 5.91 (1H, d, J=6.0 Hz), 5.46 (2H, br s), 5.40 (1H, br s), 5.21 (1H, br s), 4.63 (1H, d, J=4.4 Hz), 4.16 (1H, br s), 3.98 (1H, d, J=2.8 Hz), 3.68 (1H, d, J=12 Hz), 3.58 (1H, m); ¹³C NMR (DMSO-d₆, 100 MHz) δ 151.8, 139.5, 130.3, 129.8, 129.3, 127.3, 126.9, 126.8, 126.3, 125.7, 124.7, 124.6, 124.2, 123.5, 123.4, 122.7, 87.5, 85.5, 73.1, 70.2, 61.3; ESI-HRMS calcd. for C₂₇H₂₄N₅O₄: 482.1823, found: m/z 482.1827 [M+H]⁺.

Radioligand Binding Assays

Radioligand binding assays were performed by MDS Pharma Services Taiwan (Taipei, Taiwan) using standard binding protocols. For the binding assay of the A_(2A) receptor,⁴⁶ membrane proteins collected from HEK293 cells overexpressing the human A_(2A) receptor were incubated in reaction buffer [50 mM Tris-HCl (pH 7.4), 10 mM MgCl₂, 1 mM EDTA, and 2 U/mL adenosine deaminase] containing ³H-CGS21680 (50 nM) for 90 min at 25° C. Nonspecific binding was assessed in the presence of 50 μM adenosine-5′-N-ethylcarboxamide (NECA).

Binding assays for adenosine transporters were conducted as described earlier.⁴⁷ Membrane fractions collected from the cerebral cortex of Duncan Hartley derived guinea pigs were incubated with ³H-labeled NBTI (0.5 nM) for 30 min at 25° C. in an incubation buffer containing 50 mM Tris-HCl (pH 7.4). Nonspecific binding was assessed in the presence of 5 μM NBTI, a high-affinity inhibitor of equilibrative nucleoside transporter 1 (ENT1), which inhibits only ENT1 at 0.5 nM.⁴⁸ Reactions were terminated by filtration over GF/B glass fibers and washing with the corresponding reaction buffer.

MTT Metabolism Assay.

PC12 cells purchased from ATCC (Manassas, Va., USA) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% horse serum and 5% fetal bovine serum and incubated in a CO₂ incubator (5%) at 37° C. Survival was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) metabolism assay as described elsewhere.^(49,50) In brief, cells grown on 150-mm plates were washed three times with PBS and resuspended in DMEM. Suspended cells (1×10⁴ cells) were plated on 96-well plates and treated with or without the indicated reagent. After incubation for 24 h, MTT (0.5 mg/mL) was added to the medium and incubated for 3 h. After discarding the medium, DMSO (100 μL) was then applied to the well to dissolve the formazan crystals derived from the mitochondrial cleavage of the tetrazolium ring by live cells. The absorbance at 570/630 nm in each well was measured on a micro-enzyme-linked immunosorbent assay (ELISA) reader.

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1. A compound having an adenosine structural scaffold with variations at C-6 and C-5′ with the following structure

wherein n is 1 to 3, R¹ selected from the group consisting of (substituted)-benzene, polyarene and heterocycle, R² is selected from the group consisting of halogen, hydroxyl, alkoxy, azido, amino, (substituted)amino, amido, sulfanyl, sulfonyl, triazolyl, and cyano groups, and R³ is selected from the group consisting of (substituted)carbonyl, carboxylate, (substituted)carbamide, cyano, (substituted)alkynyl, and (substituted)tetrazole groups.
 2. The compound according to claim 1 having the structure of

wherein the (substituted) heterocycle contains 5- or 6-membered rings and the fused heterocycle contain nitrogen, oxygen or sulfur heteroatoms and the substituents is selected from the group consisting of hydrogen, halogen (fluorine, chlorine, bromine and iodine), hydroxy, alkyl (1 to 6 carbons), trifluoromethyl, and (substituted)phenyl group.
 3. The compound of claim 2 where the heterocycle is selected from the group consisting of pyrrole, furan, thiophene, pyridine, piperidine, piperazine, indole, benzofuran, benzothiophene, and quinoline.
 4. The compound according to claim 1 having the structure of

The aromatic ring is selected from the group consisting of benzene, naphthalene, anthracene, phenanthrene, and pyrene; when n=1, the benzyl group may optionally have substituents selected from the group consisting of halogen (fluorine, chlorine, bromine and iodine), alkyl (methyl, ethyl, propyl, butyl and trifluoromethyl), phenyl, hydroxy, alkoxyl (OR where R═CH₃, C₂H₅, C₃H₇ and C₄H₉), amino (NRR′ where R, R′ represent H, CH₃, C₂H₅, C₃H₇, C₄H₉ and phenyl), amido (NHCOR where R═CH₃, C₂H₅, C₃H₇ and C₄H₉), nitro, sulfonate, alkanoyl (COR where R═H, CH₃, C₂H₅, C₃H₇, C₄H₉ and phenyl), and carboxylate (CO₂R where R═H, CH₃, C₂H₅, C₃H₇, C₄H₉ and phenyl).
 5. The compound according to claim 1 having the structure of

wherein R³ is selected from the group consisting of hydrogen, alkyl (1 to 4 carbons), and (substituted)phenyl groups.
 6. The compound according to claim 1 having the structure of

wherein R⁴ is selected from the group consisting of hydrogen, halogen (fluorine, chlorine, bromine and iodine), hydroxy, alkyl (1 to 6 carbons), trifluoromethyl, and (substituted) phenyl group.
 7. The compound according to claim 1 having the structure of

wherein R⁵ is selected from the group consisting of hydrogen and alkyl (1 to 4 carbons) groups.
 8. The compound according to claim 4 having the structure of

wherein R is selected from the group consisting of H, halogen (F, Cl, Br, and I), alkyl (1 to 4 carbons, trifluoromethyl, phenyl, hydroxyl, alkoxy (1 to 4 carbons), (substituted)amino, (substituted)amido, nitro, sulfonate, carbonyl, and carboxylate groups located at ortho-, meta- or para-positions, and wherein x is 1 to
 5. 9. A method for treating neurodegenerative disease, comprising administering to a subject in need thereof an effective amount of at least one of the compounds of claim
 1. 10. The method of claim 9, wherein the neurodegenerative disease is a protein-misfolding disease.
 11. The method of claim 9 wherein the neurodegenerative disease is Huntington's disease.
 12. A composition comprising a compound of claim 1 and a pharmaceutically acceptable carrier.
 13. A compound having the following structure:


14. A method for treating neurodegenerative disease, comprising administering to a subject in need thereof an effective amount of at least one of the compounds of claim
 13. 15. The method of claim 14 wherein the neurodegenerative disease is an protein-misfolding disease.
 16. The method of claim 14 wherein the neurodegenerative disease is Huntington's disease. 